Spore stain free#

The warming time greatly affect the dye to penetrate the walls of bacterial spores, this can be seen in the results with various concentration at pH 10, indicates that the not long warm-up time 3 and 4 minutes, bacterial spores are not stained, while in the longer heating time is 5 minutes bacterial spores stained. The size, morphology, and location of the spore differ between bacterial species and can be used to help identify bacteria (Figure 1.8(C)). Special staining is required to stain the spore and distinguish it from the bacterial cell (Figure 1.8(B)). The safranin counterstain is used on the slide to give color to the vegetative. Spores are difficult to stain due to their thick cell wall.

Spore stain free#

The water acts as a decolorizer for the vegetative cells, but the stain is not released by the endospores and free spores. Once the heating period is complete, the slide is washed gently with tap water. Simple stains are used to stain whole cells or to stain specific cellular components. The all treatments staining have been effect on bacterial spores staining results. heating step stains the vegetative cells and the endospores. SIMPLE STAINING A staining method that uses only a single dye that which does not differentiate between different types of organisms There is only a single staining step and everything is stained with the same color. Place the wire stain rack over the beaker which now has steam coming up from the boiled water. Put a beaker of water on the hot plate and boil until steam is coming up from the water. The alternative staining used is Methylene Blue solution (0,5 %, 0,7%, and 1% concentration) with pH variation (10, 11, and 12), and varyous heating time (3, 4, and 5 minutes). THE PROCEDURE Prepare a smear of the Bacillus species-air-dry and heat-fix. In this research, it have found the alternative staining that can replace Malachite Green solution in spore bacterial stain. It is an alkaline substance staining that can staining the spore bacteria. Baso Spore Stain is a very useful tool for bacteria. Endospores are protective structures within an. Spores, which have strong refraction, can be observed under light microscope when properly stained. A method of endospores staining is Schaeffer Fulton method that used Malachite Green. Endospore staining is a special staining technique, to observe bacterial spores, where the spores take the color of the primary stain Malachite green. This test is selected to detect endospores by staining and morphological examination of microorganisms. The bacterial endospores need a staining which can penetrate wall thickness of spore bacteria. This is the basis of several spore stains such as the Schaeffer-Fulton staining method which also differentiates the spores from sporangia and vegetative. Because the endospore coat is so tough, steam is used to.

Endospores staining is the type of staining to recognize the presence spore in bacterial vegetative cells. The presence of endospores in a bacterial culture can be detected by staining with malachite green.